Journal: American Journal of Cancer Research

Article Title: PBX4 functions as a potential novel oncopromoter in colorectal cancer: a comprehensive analysis of the PBX gene family

doi:

Figure Lengend Snippet: Study methodology flow chart of in vitro PBX4 overexpression in HCT116 cell line.

Article Snippet: In vitro gene expression modulation Study design and protocol For the in vitro studies, the human HCT116 colon adenocarcinoma cell line was acquired from the American Type Culture Collection (ATCC, USA) which has low endogenous PBX4 expression.

Techniques: In Vitro, Over Expression

Journal: American Journal of Cancer Research

Article Title: PBX4 functions as a potential novel oncopromoter in colorectal cancer: a comprehensive analysis of the PBX gene family

doi:

Figure Lengend Snippet: Impact of PBX4 gene overexpression on HCT116 cell proliferation in vitro. (A) HCT116 cell proliferation was measured as relative fluorescence (RFU) after PBX4 overexpression. Comparison groups were pPBX4 (overexpressing) and the control group which was transfected with an empty vector. Y-axis represents time points post-transfection. Data are presented as mean ± standard error of the mean (SEM) from 4 independent experiments containing 8-12 technical replicates and the values are shown in (B) alongside the P-values. (C) Relative PBX4 expression in HCT116 negative control group and in the overexpressing group from three independent experiments analysed in triplicates. (D) Western blot evaluation of PBX4 overexpression in HCT116 cells. NC represents the group transfected with an empty vector, UT represents the untransfected group and pPBX4 is the overexpressing group, The bottom band shows the expression of β-actin which was used as a loading control. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: In vitro gene expression modulation Study design and protocol For the in vitro studies, the human HCT116 colon adenocarcinoma cell line was acquired from the American Type Culture Collection (ATCC, USA) which has low endogenous PBX4 expression.

Techniques: Over Expression, In Vitro, Fluorescence, Comparison, Control, Transfection, Plasmid Preparation, Expressing, Negative Control, Western Blot

Journal: American Journal of Cancer Research

Article Title: PBX4 functions as a potential novel oncopromoter in colorectal cancer: a comprehensive analysis of the PBX gene family

doi:

Figure Lengend Snippet: Impact of PBX4 overexpression on EMT and angiogenesis markers in vitro. RNA fold change expression of EMT-related and angiogenesis markers in HCT116 overexpressing PBX4 vs. empty vector (control) group. Data are presented as mean ± SEM from three independent experiments analysed in triplicates. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. VIM: Vimentin; CDH1: Cadherin-1; CDH2: Cadherin-2; ZEB1: Zinc Finger E-Box Binding Homeobox 1; ZEB2: Zinc Finger E-Box Binding Homeobox 2; SNAI1: Snail Family Transcriptional Repressor 1; SNAI2: Snail Family Transcriptional Repressor 2; TWIST: Twist Family BHLH Transcription Factor 1; VEGFA: Vascular Endothelial Growth Factor A.

Article Snippet: In vitro gene expression modulation Study design and protocol For the in vitro studies, the human HCT116 colon adenocarcinoma cell line was acquired from the American Type Culture Collection (ATCC, USA) which has low endogenous PBX4 expression.

Techniques: Over Expression, In Vitro, Expressing, Plasmid Preparation, Control, Binding Assay

Journal: American Journal of Cancer Research

Article Title: PBX4 functions as a potential novel oncopromoter in colorectal cancer: a comprehensive analysis of the PBX gene family

doi:

Figure Lengend Snippet: Putative PBX4 regulators from Cistrome DB

Article Snippet: In vitro gene expression modulation Study design and protocol For the in vitro studies, the human HCT116 colon adenocarcinoma cell line was acquired from the American Type Culture Collection (ATCC, USA) which has low endogenous PBX4 expression.

Techniques: Gene Expression, Binding Assay, Sequencing, Expressing, Cell Differentiation, Variant Assay

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Development of novel ALOX15 inhibitors combining dual machine learning filtering and fragment substitution optimisation approaches, molecular docking and dynamic simulation methods

doi: 10.1080/14756366.2024.2301756

Figure Lengend Snippet: Structure, Libdock and CDOCKER energy scores of the three lead molecules and the control inhibitor i472.

Article Snippet: Libdock module of Discovery Studio was utilised to conduct a virtual screening of 6,140 compounds from the Targetmol FDA compound database, with the Homo sapiens ALOX15 protein model (P16050-LOX15_HUMAN) downloaded from the Alpha fold website as the receptor structure.

Techniques: Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Development of novel ALOX15 inhibitors combining dual machine learning filtering and fragment substitution optimisation approaches, molecular docking and dynamic simulation methods

doi: 10.1080/14756366.2024.2301756

Figure Lengend Snippet: Novel molecules obtained by i472 fragment replacement optimisation.

Article Snippet: Libdock module of Discovery Studio was utilised to conduct a virtual screening of 6,140 compounds from the Targetmol FDA compound database, with the Homo sapiens ALOX15 protein model (P16050-LOX15_HUMAN) downloaded from the Alpha fold website as the receptor structure.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Development of novel ALOX15 inhibitors combining dual machine learning filtering and fragment substitution optimisation approaches, molecular docking and dynamic simulation methods

doi: 10.1080/14756366.2024.2301756

Figure Lengend Snippet: Structural and target interaction information for OSI-930 and its fragment replacement optimised molecule osia - osif.

Article Snippet: Libdock module of Discovery Studio was utilised to conduct a virtual screening of 6,140 compounds from the Targetmol FDA compound database, with the Homo sapiens ALOX15 protein model (P16050-LOX15_HUMAN) downloaded from the Alpha fold website as the receptor structure.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Development of novel ALOX15 inhibitors combining dual machine learning filtering and fragment substitution optimisation approaches, molecular docking and dynamic simulation methods

doi: 10.1080/14756366.2024.2301756

Figure Lengend Snippet: Structural and target interaction information for GS-444217 and its fragment substitution optimised molecule gsa–gsh.

Article Snippet: Libdock module of Discovery Studio was utilised to conduct a virtual screening of 6,140 compounds from the Targetmol FDA compound database, with the Homo sapiens ALOX15 protein model (P16050-LOX15_HUMAN) downloaded from the Alpha fold website as the receptor structure.

Techniques:

Journal: Journal of Neuroscience

Article Title: Central Vagal Afferent Endings Mediate Reduction of Food Intake by Melanocortin-3/4 Receptor Agonist

doi: 10.1523/jneurosci.1121-14.2014

Figure Lengend Snippet: Figure 4. Attenuation of MTII-induced synapsin I phosphorylation and reduction of food intake by the PKA inhibitor KT5720. A, Left, Representative fluorescent images of hindbrain pSynimmunoreactivityinratsthatreceivedafourth-ventricleinjectionofKT5720(10pmol)or vehiclebeforefourth-ventricleadministrationofsalineorMTII(50pmol).Right,Quantification of pSyn immunoreactivity in the DMV and NTS for this experiment (n 4 per treatment). B, Cumulative food intake in overnight-fasted rats (n 16) that received an NTS injection of KT5720(1pmol)orvehiclesolutionbeforeNTSinjectionofsalineorMTII(50pmol).Bargraphs representaveragecumulativefoodintakebetween0and4h,and4–24hafterinjection.Data are means SEM, *, Values significantly different (p 0.05) from vehicle/saline control treatment. *#, Values that are significantly different (p 0.05) from vehicle/saline and vehi- cle/MTII treatments.

Article Snippet: Sixteen rats with cannulas aimed for the NTS were used in a crossover, counterbalanced design to study the effect of the PKA inhibitor KT5720 (Tocris Bioscience) on MTII-induced reduction of food intake.

Techniques: Phospho-proteomics, Injection, Saline, Control